Journal: STAR Protocols

Article Title: Protocol for 3D-guided sectioning and deep cell phenotyping via light sheet imaging and 2D spatial multiplexing

doi: 10.1016/j.xpro.2025.104296

Figure Lengend Snippet: 3D light sheet and 2D multi-cyclic imaging data comparison (Mouse Glioblastoma) (A) Imaris 3D surface rendering of autofluorescence (cyan) and glioblastoma target cells stained with anti-GFP-Alexa Fluor 647 nanobody (red). (B) Imaris 3D surface rendering of autofluorescence (cyan) and glioblastoma target cells stained with anti-GFP-Alexa Fluor 647 nanobody (red) with target plane in yellow. (C) Optical section of target plane of interest. (D) Fluorescence image of physical cryosection. (E) MICS image of section shown in D. (F) MICS image indicating anti-GFP-Alexa Fluor 647 nanobody (red) staining. (G) Magnified merged four color multiparameter MICS image with anti-EGFR (magenta), anti-GFAP (green), anti-NeuN (blue), anti-CD146 (yellow). (H–P) Nine exemplary MICS images with merges of anti-GFP-Alexa Fluor 647 nanobody staining (red) and antibody-conjugates against EGFR (H), Neurofilament (I), Nestin (J), GFAP (K), CD44 (L), CD146 (M), NeuN (N), EphA2 (O) and GLAST (P) (gray) (see “Antibodies”). Scale bars: (A–F) 500 μm; (G) 50 μm; (H–P) 500 μm.

Article Snippet: CD44 antibody, anti-mouse, APC, REAfinity , Miltenyi Biotec B.V. & Co. KG , Cat# 130-119-121 RRID: AB_2751628.

Techniques: Imaging, Comparison, Staining, Fluorescence

Journal: iScience

Article Title: KRT15 identified by scRNA-Seq and machine learning as stemness regulator and prognostic biomarker in ESCC

doi: 10.1016/j.isci.2026.115020

Figure Lengend Snippet: KRT15 is enriched in ESCC tumor spheroids and positively correlates with the stemness marker CD44 (A) Stem cell enrichment experiments performed in four cell lines, with images captured over time. (B and C) qPCR analysis of CD44 and KRT15 mRNA expression in tumorspheres versus adherent cells from ECA109, KYSE180, KYSE150, and TE1 cell lines. n = 3, statistical analysis performed using an unpaired t test. Data are represented as mean ± SEM. ∗ p < 0.05, ∗∗ p < 0.01, ∗∗∗ p < 0.001, and ∗∗∗∗ p < 0.0001. (D) Western blot analysis of CD44 and KRT15 protein expression in tumor spheres compared to adherent cells in ECA109 and KYSE180 cell lines.

Article Snippet: CD44 Polyclonal antibody , Proteintech , Cat#15675-1-AP; RRID: AB_2076198.

Techniques: Marker, Expressing, Western Blot

Journal: iScience

Article Title: KRT15 identified by scRNA-Seq and machine learning as stemness regulator and prognostic biomarker in ESCC

doi: 10.1016/j.isci.2026.115020

Figure Lengend Snippet: KRT15-mediated stemness in ESCC cells is associated with MYC pathway activation and involves upregulation of stemness markers (A) The significantly enriched hallmark pathways identified by GSEA of differentially expressed genes in cluster 0. (B) The GSEA enrichment plot demonstrates a significant enrichment of the MYC_TARGETS_V1 gene set. (C and D) WB results showed that when KRT15 was overexpressed, c-MYC protein levels were synergistically upregulated alongside stemness markers (CD44, SOX2, OCT4); conversely, when KRT15 was knocked down, all these markers exhibited a downward trend.

Article Snippet: CD44 Polyclonal antibody , Proteintech , Cat#15675-1-AP; RRID: AB_2076198.

Techniques: Activation Assay